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Bacterial expression of G-protein coupled receptor CB2 fusion protein
- Nicole L WomackCollege of Natural Resources & SciencesUndergraduate Student
- Hannah CornwellUndergraduate Student
- David LopezUndergraduate Student
- Dylan MorrisUndergraduate Student
The G-protein coupled receptor (GPCR), CB2, is part of a widespread neuromodulatory system found in all animals and is suspected to play a role in both CNS and immune system functions, yet it is a relatively understudied. We demonstrated a successful method for the expression and purification of the GPCR CB2 protein from BL21(DE3) pLysS competent cells. This method involves the culturing of an E. coli BL21(DE3) pLysS MBP-CB2-6xHis cell line, induction of CB2 receptor expression via IPTG, and the harvest and isolation of expressed CB2 proteins via Ni-NTA Immobilized Metal Affinity Chromatography. This method was proven to be successful based on SDS-page and spectrophotometric analyses.
Bake the World a Better Place: 501(c)3 Development
- Jeff RichSocial WorkGraduate Student
Bake the World a Better Place is a non-profit organization based out of Denver, Colorado that aims to holistically improve the community. Presently, the organization engages in job training and job placement of marginalized populations such as those experiencing homelessness. Additionally, continuing collaborations are being made with local hotels to assist with housing individuals during the job training process. Products made during the classes are shared with individuals living on the streets. Through building community partnerships between business, social service agencies, and vulnerable populations, each entity is taking an active role in helping improve their community.
Baker Cypress Seed Viability in Relation to Cone Age
- Cooper HarrisForestry; Resource Management and ProtectionUndergraduate Student
- Taylor KnottForestry; Resource Management and ProtectionUndergraduate Student
Hesperocyparis bakeri, better known as Baker cypress, is a serotinous species which suffers from low seed viability. Previous research has shown that Baker cypress seed viability begins to decline with cone age and thus the low overall viability of Baker cypress cones is not unexpected (Milich et al., 2012). However, little is known about the cone age at which seed viability begins to decrease. We investigated seed viability in relation to cone age by sampling twenty branches from five Baker cypress trees located in Burney, Shasta County in north-eastern California. Seeds were scarified and placed in a 1% tetrazolium red solution for 18 hours, cut longitudinally to determine viability.
Balance and Wellness in First Responder Agencies
- Catherine MunseeSocial WorkGraduate Student
This project fulfills the requirements for the Masters degree in Social Work. The design of the project is informed by a relational worldview, specifically the work of Terry Cross who suggests that a circular experience of the world intersects with more linear representations of reality. It is at the places of intersection that opportunities for dialogue occur and the intent of the project is to make the experiences of first responders visible to those who do not have access to this knowledge and experience. The creative work is the construction of an empathic bridge between the community and first responders through poetry in honor of the belief that we are all connected.
Balance, Mobility, and Fall Risk in Indigenous Rural-Dwelling Older Adults and Urban Dwelling Older Adults in Humboldt and Del Norte County
- Andre BouweraertsKinesiology DepartmentGraduate Student
By 2030, 20% of the U.S. population will be over the age of 65. Among this age group, falls are the leading cause of injuries, hospitalizations, and fatalities in the United States. Within California, rural community dwelling communities have higher rates of falls than urban. Indigenous populations fall more than the national average (34-40.3% vs. 30%), suggesting the rates of falls, and thus physiological declines, may be greatest among Indigenous rural community-dwelling populations. The purpose of this study is to examine intrinsic, extrinsic, and the incidence of falls among rural dwelling Indigenous older adults and urban dwelling older adults in Humboldt and Del Norte Counties.
Barn owl nest box selection on a pistachio farm in the Central Valley
- Jaime CarlinoWildlifeUndergraduate Student
Overabundant populations of rodents in agricultural areas cost California farmers 5.3% to 8.8% in profits annually. Installing nest boxes to encourage the presence of rodent predators such as barn owls (Tyto alba) is a form of biocontrol that has the potential to limit rodent activity in agricultural settings. Previous works identified the spatial scales of nest box selection on vineyards in Napa and Sonoma Counties which are rather heterogenous agricultural landscapes. Determining at which spatial scale barn owls select nest boxes in the Central Valley can inform how nest boxes should be constructed and where they are best placed to encourage occupancy in homogenous agricultural landscapes.
Barriers to LGBTQ+ Transition Age Youth in Humboldt County
- Eugene WilliamsSocial WorkGraduate Student
My community partner is the Humboldt County Transition Age Youth Collaboration (HCTAYC). The project is a preliminary research project that looks at the barriers in systems serving the LGBTQ+ transition age youth (16-26) population in Humboldt County. The outcomes from the research are the base for policy recommendations that will be used at HCTAYC. The results from the research done will address the specific question: “How are systems serving the LGBTQ+ transition age youth community in Humboldt County?”
Bat Activity within Urban and Rural Landscape in Arcata, CA
Gonzalo Ayala, Wildlife Undergraduate Student
College of Natural Resources & SciencesBats are found in urban and natural habitats, although their presence and behavior may differ depending on the environment. Bats can also be affected by various anthropogenic factors such as land conversion, and displacing their establishment. I determine the activity of 3 bat species: California myotis, silver-haired bat, and Mexican free-tailed bat, within urban and natural landscapes found throughout Arcata, CA. Urban landscapes were determined by the presence of buildings in every cardinal direction. This research approach can help determine how bats use valuable habitats which can be incorporated into urban planning.
Bayside Corners
- Jasson FloresGeographyUndergraduate Student
- Brooks SpencerGeographyUndergraduate Student
Bayside Corners is a Nonprofit Organization which seeks to help foster community building in the community of Bayside.
Beau Pre Golf Club presents: College Night
Brenden Barry, School of Applied Health Undergraduate Student
College of Professional StudiesThe event was referred to as College Night or College Golf Night, but all the flyers said Beau Pre Golf Club presents: College Night. The goal for the event was to get students out recreating at a local recreation facility and to introduce my fellow students to the great game of golf in the cheapest way possible, FREE!